TABLE 1.
Oligonucleotides used for the construction of SalI sites in the mntB gene of Synechocystis sp. strain PCC 6803
| Oligonu-cleotide | Sequence (5′ to 3′)a | Site of fusion in MntB protein |
|---|---|---|
| B1 | CAAAGGCTCCGTCGACCAATGCCAAAA | 13W |
| B2 | AGGCGCTCACCCAAGTCGACCGGATTAA | 25R |
| B3 | CCATTAAGTCGACCAACCTTTGAGA | 49W |
| B4 | GAATAGTCGACGCATAGGCCAGCACTA | 69A |
| B5 | TGCCAATGACTGCAGTCGACTTTAACCG | 99K |
| B6 | TGGAATAGGTCGACTTACTAGGAA | 124S |
| B7 | CAAAACAAGAGCAGGTCGACTTGCG | 163K |
| B8 | TGCGTAGTCGACGCCTTGGCATGGTTT | 177A |
| B9 | TTGTAAAGTCGACACAATGGTGAGG | 198V |
| B10 | AAGCATAGTCGACTCAAAGCGATCGCT | 227F |
| B11 | CCGGTCGACACATCAAAGTGATAGC | 254V |
| B12 | CCACAGTCGACTTTTCATCGTCTTCC | 297E |
a
SalI sites are underlined. Boldface nucleotides indicate introduced mutations.