Table 2.
Genetic features deduced from whole genome sequencing of the hypervirulent ST147 K. pneumoniae isolates
| Locus | Locationa | Positionsb | Description/phenotype | |
|---|---|---|---|---|
| IncFIB(Mar) | Plasmid | 646 | 208 | Replicon |
| IncR | Plasmid | 35 322 | 35 072 | Replicon |
| aac(6’)-Ib | Plasmid | 52 273 | 52 878 | Amikacin, gentamicin, kanamycin resistance |
| aadA1 | Plasmid | 52 948 | 53 736 | Streptomycin resistance |
| bla OXA-9 | Plasmid | 53 781 | 54 620 | Ampicillin resistance |
| bla TEM-1A | Plasmid | 55 320 | 56 180 | Ampicillin resistance |
| rmpA | Plasmid | 162 998 | 163 630 | Enhancer of cps synthesis |
| iucA | Plasmid | 180 751 | 182 541 | Hydroxamate siderophore aerobactin |
| iucB | Plasmid | 182 542 | 183 489 | |
| iucC | Plasmid | 183 489 | 185 222 | |
| iucD | Plasmid | 185 226 | 186 503 | |
| iutA | Plasmid | 186 585 | 188 786 | |
| rmpA2 | Plasmid | 193 677 | 194 314 | Enhancer of cps synthesis |
| aph(3′)-Ia | Plasmid | 240 342 | 239 527 | Kanamycin resistance |
| mph(A) | Plasmid | 242 200 | 241 298 | Erythromycin, azithromycin resistance |
| sul1 | Plasmid | 249 689 | 248 850 | Sulfisoxazole resistance |
| dfrA5 | Plasmid | 250 687 | 250 214 | Trimethoprim resistance |
| mph(E) | Plasmid | 258 500 | 257 616 | Erythromycin, azithromycin resistance |
| msr(E) | Plasmid | 260 031 | 258 556 | Erythromycin, azithromycin resistance |
| armA | Plasmid | 263 103 | 262 330 | Amikacin, gentamicin, kanamycin, streptomycin resistance |
| sul2 | Plasmid | 265 585 | 266 400 | Sulfisoxazole resistance |
| IncHI1B | Plasmid | 279 395 | 278 826 | Replicon |
| bla CTX-M-15 | Plasmid | 369 187 | 370 062 | Ampicillin, ceftriaxone resistance |
| mrkA | Chromosome | 876 941 | 877 549 | Type 3 fimbriae synthesis |
| mrkB | Chromosome | 877 645 | 878 346 | |
| mrkC | Chromosome | 878 358 | 880 844 | |
| mrkD | Chromosome | 880 835 | 881 830 | |
| mrkF | Chromosome | 881 844 | 882 479 | |
| mrkJ | Chromosome | 882 514 | 883 230 | |
| mrkH | Chromosome | 883 964 | 884 668 | |
| bla CTX-M-15 | Chromosome | 1 597 014 | 1 597 889 | Ampicillin, ceftriaxone resistance |
| bla CTX-M-15 | Chromosome | 1 671 695 | 1 672 570 | Ampicillin, ceftriaxone resistance |
| fyuA | Chromosome | 1 863 698 | 1 865 719 | Yersiniabactin receptor resistance |
| ybtE | Chromosome | 1 865 850 | 1 867 427 | Yersiniabactin biosynthetic operon |
| ybtT | Chromosome | 1 867 431 | 1 868 234 | |
| ybtU | Chromosome | 1 868 231 | 1 869 331 | |
| irp1 | Chromosome | 1 869 328 | 1 878 819 | |
| irp2 | Chromosome | 1 878 907 | 1 885 014 | |
| ybtA | Chromosome | 1 885 205 | 1 886 164 | |
| ybtP | Chromosome | 1 886 421 | 1 888 133 | |
| ybtQ | Chromosome | 1 888 120 | 1 889 922 | |
| ybtX | Chromosome | 1 889 915 | 1 891 195 | |
| ybtS | Chromosome | 1 891 223 | 1 892 527 | |
a
Plasmid refers to location in the single large plasmid of 382 377 bp with FIB(Mar)-R-HI1B replicons, identified in the PA strain, carrying both virulence and resistance genes.
b
Nucleotide positions refers to start and stop codon of each gene of the plasmid and chromosome complete circular sequences, respectively (Bioproject PRJNA746592; Accession no.s CP084984–CP084985).