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. 2022 Aug 12;36(9):e22476. doi: 10.1096/fj.202200045R

FIGURE 3.

FIGURE 3

Heightened development of circulating human natural killer (NK) cells in NOD‐scid IL2rγ null (NSG)‐Tg(Hu‐IL15) mice. NSG (n = 15) or NSG‐Tg(Hu‐IL15) (n = 15) mice 6 to 8 weeks of age were irradiated (200 cGy) and injected IV with 100 000 CD34+ hematopoietic stem cell (HSC) derived from CD3‐depleted UCB as described in the Materials and Methods. Mice were bled at the indicated time points post‐injection and blood analyzed by flow cytometry for frequencies of human NK cells. (A) Representative flow plot of NK cells at 12 weeks post‐HSC injection. The human NK cells were gated on hCD45+/CD3−/CD33−/CD20−/CD7+ cells. The proportions of human (B) CD56dim/CD16+, (C) CD56bright/CD16−, and (D) CD16+ human NK cells are shown. (E) Total number of CD56dim/CD16+ NK cells per μl of blood is shown. (F) Total number of CD56‐bright NK cells per μl of blood is shown. Each point represents an individual mouse. For statistical analysis, HSC‐engrafted NSG‐Tg(Hu‐IL15) mice were compared with HSC‐engrafted NSG mice; *p < .05, **p < .01, ***p < .001, ****p < .0001. The results are representative of three independent experiments.