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. 2022 Aug 12;36(9):e22476. doi: 10.1096/fj.202200045R

FIGURE 5.

FIGURE 5

Phenotypic profiling of natural killer (NK) cells in NOD‐scid IL2rγ null (NSG)‐Tg(Hu‐IL15) mice. NSG or NSG‐Tg(Hu‐IL15) mice 6 to 8 weeks of age were irradiated (200 cGy) and injected IV with 100 000 CD34+ hematopoietic stem cell (HSC) derived from CD3‐depleted UCB as described in the Materials and Methods. At 12 weeks post‐human HSC engraftment, blood was analyzed for human CD56dim/CD16+ NK cell surface receptors by flow cytometry. Data are displayed as tSNE plots; natural cytotoxicity receptors, including NKp46 (A) and NKp30 (B); NKG family molecules, including NKG2C (C), NKG2D (D), NKG2A (E), and CD94 (F); KIRs, including KIR3DL1 (G), KIR2DL2/L3 (H), and KIR2DS4 (I); and maturation markers CD8 (J) and CD57 (K). The tSNE 2D scatter plots show the flow cytometry analysis of expression levels (red, high; blue, and low) of the surface markers. Each point represents an individual mouse. For statistical analysis, HSC‐engrafted NSG‐Tg(Hu‐IL15) mice were compared with HSC‐engrafted NSG mice; *p < .05, **p < .01, ***p < .001, ****p < .0001. The data are representative of two independent experiments.