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DEAE-Sephacel anion exchange column chromatography profiles of [U-14C] glucose labeled OPGs from strains DF214 (mdo+), NFB734 (mdoB), NFB1933 (mdoB mdoC), and NFB1919 (mdoC). Ionic strength was increased by steps of 0.05 M NaCl at fractions indicated by the arrows. Fractions (4 ml) were collected and aliquots were counted. Peak I, neutral glucans; peaks II to V, anionic glucans. The number on the right of each peak refers to the percentage of the total for the corresponding subfraction.
