TABLE 1.

Bacterial strains and phage used in this study

Strain or phage	Relevant genotype	Construction, source, or reference
Bacterial strains
JM83	ara Δ(lac-pro) thi rpsL (φ80 ΔlacZ ΔM15)	32
DF214	his pgi::Mu Δ(zwf-edd)1 eda-1 rpsL	D. Fraenkel via E. P. Kennedy
PT227	F−thr-1 leuB6 Δ(gpt-proA)62 hisG4 argE3 thi-1 ara-14 lacY1 galK2 xyl-5 mtl-1 mdoB214::Tn10 rpsL31 glnV44	16
NFB732	JM83 mdoB214::Tn10	JM83 + P1 (PT227)
NFB734	DF214 mdoB214::Tn10	DF214 + P1 (PT227)
NFB1864	JM83 mdoB214::Tn10 mdoC1::Tn5	Tn5 mutant
NFB1897	JM83 mdoB214::Tn10 mdoC1::Tn5	NFB732 + P1 (NFB1864)
NFB1919	DF214 mdoC1::Tn5	NFB732 + P1 (NFB1864)
NFB1933	DF214 mdoB214::Tn10 mdoC1::Tn5	NFB734 + P1 (NFB1864)
Bacteriophage
Phage λNK467	b221 rex::Tn5 cI857 Oam29 Pam80	N. Kleckner via A. Pugsley