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. 2022 May 25;68(8):1042–1052. doi: 10.1093/clinchem/hvac081

Fig. 2.

Fig. 2.

Detection of Iota (B.1.526, New York) SARS-CoV-2 variant by fragment analysis. (A), Expected deletions present in Gamma, (P.1, Brazil), Beta (B.1.351, South Africa), and Iota (B.1.526, New York) VOCs compared to WT; (B), Electrophoretograms representative of clinical WT SARS-CoV-2 infection and one with SARS-CoV-2 exhibiting a 9-bp deletion in ORF1A characteristic of Beta/Gamma/Iota. Integrated genome viewer (IGV) visualization of the WGS reads confirming the presence of the ORF1A:del3675_3677 (C), which is found in Beta/Gamma/Iota. Lack of the N501Y (D) and presence of the E484K (E) mutation along with other mutations led to classification of this SARS-CoV-2 strain as Iota.