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. 2022 May 25;68(8):1042–1052. doi: 10.1093/clinchem/hvac081

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Fig. 3. — Allele-specific primers for S:N501Y and S:E484K. (A), Scheme for designing allele-specific primers with destabilizing mutations; (B), Electrophoretograms of 4 combinations of E484WT- and E484K-labeled primers with (C) graphs quantifying fluorescence intensity in WT specimens, (D) E484K mutated specimens, and (E) the ratio of the 2. (F), Electrophoretogram of signal from N501 and N501Y allele-specific primers; and (G), graphical representation of FAM and HEX signal in response to WT and N501Y specimens.