FIG. 2.

Structural alignment of RRs with a high degree of similarity to YycF. The conserved residues necessary for proper phosphotransfer (56) in the regulatory domain are boxed and highlighted with an inverted triangle. The location of the amino acid substitution (E63K) in the variant form of the RR is highlighted by a star. Abbreviations: Bsu, B. subtilis; Sau, S. aureus; Efa, E. faecalis; Spn, S. pneumoniae; Spy, Streptococcus pyogenes. The S. pneumoniae (type 4) unpublished genomic sequence data used to identify the YycF orthologue were provided by The Institute for Genomic Research (25a). The E. faecalis sequence (strain V583) was obtained by designing PCR primers based on the unpublished genomic sequence data provided by The Institute for Genomic Research (25a) and then resolving frameshifts by sequencing PCR-derived amplicons from strain ATCC 29212. The S. pyogenes sequence (strain M1 GAS) was provided by the University of Oklahoma (44a).