REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
COVA1-18	Brouwer et al., 2020	MT599837.1, MT599921.1
SKOT-9	Ohnishi et al., 2005	N/A
HRP-conjugated goat anti-human IgG	Southern Biotech	Cat# 2040-05; RRID: AB_2795644
FcR Blocking Reagent, human	Miltenyi Biotec	Cat# 130-059-901; RRID: AB_22892112
PerCP anti-human CD3 Antibody (clone UCHT1)	BioLegend	Cat# 300428; RRID: AB_893298
Brilliant Violet 605™ anti-human CD4 Antibody (clone OKT4)	BioLegend	Cat# 317438; RRID: AB_11218995
Alexa Fluor® 700 anti-human CD8a Antibody (clone RPA-T8)	BioLegend	Cat# 301028; RRID: AB_493745
APC/Cyanine7 anti-human CD45RA Antibody (clone HI100)	BioLegend	Cat# 304128; RRID: AB_10708880
FITC anti-human CD69 Antibody (clone FN50)	BioLegend	Cat# 310904; RRID: AB_314839
APC anti-human CD137 (4-1BB) Antibody (clone 4B4-1)	BioLegend	Cat# 309810; RRID: AB_830672
BV786 Mouse Anti-Human CD183 (clone 1C6/CXCR3)	BD Biosciences	Cat# 741005; RRID: AB_2740628
BV421 Rat Anti-Human CXCR5 (CD185) (clone RF8B2)	BD Biosciences	Cat# 562747; RRID: AB_2737766
Brilliant Violet 650™ anti-human CD196 (CCR6) Antibody (clone G034E3)	BioLegend	Cat# 353426; RRID: AB_2563869
Biological samples
SARS-CoV-2-infected convalescent patient blood sample	Tokyo Shinagawa Hospital and Tokyo Center Clinic	N/A
Chemicals, peptides, and recombinant proteins
Vacutainer CPT tube	BD Biosciences	362761
SARS-CoV-2 recombinant RBD protein	In-house, Moriyama et al. (2021)	MN994467
SARS-CoV-2 recombinant N protein	In-house, this paper	MN994467
TALON® Metal Affinity Resin	Clontech	635653
Ni-NTA agarose	Qiagen	30230
Bovine serum albumin	Sigma-Aldrich	A2153
Tween-20	Fujifilm Wako Pure Chemicals	167-11515
Can Get Signal Immunoreaction Enhancer Solution 2	TOYOBO	NKB-301
OPD substrate	Sigma-Aldrich	P8287
RPMI-1640 with L-glutamine and phenol red	Fujifilm Wako Pure Chemicals	189-02025
Penicillin/streptomycin	Thermo Fisher Scientific	15140-122
GlutaMAX Supplement	Thermo Fisher Scientific	35050061
PepMix™ SARS-CoV-2 (Spike Glycoprotein)	JPT Peptide Technologies GmbH	PM-WCPV-S-1
PepMix™ SARS-CoV-2 (NCAP)	JPT Peptide Technologies GmbH	PM-WCPV-NCAP-2
PepMix™ SARS-CoV-2 (S-RBD)	JPT Peptide Technologies GmbH	PM-WCPV-S-RBD-1
PepMix™ SARS-CoV-2 (S-RBD B.1.351)	JPT Peptide Technologies GmbH	PM-SARS2-RBDMUT02-1
PepMix™ SARS-CoV-2 (S-RBD B.1.429/Epsilon)	JPT Peptide Technologies GmbH	PM-SARS2-RBDMUT04-1
Dimethyl sulfoxide	Sigma-Aldrich	D2650
LIVE/DEAD Fixable Aqua Dead Cell Stain Kit	Thermo Fisher Scientific	L34965
Critical commercial assays
Elecsys Anti-SARS-CoV-2	Roche Diagnostics	518316181
Expi293 expression system	Thermo Fisher Scientific	A29133
Experimental models: Cell lines
Expi293F™ Cells	Thermo Fisher Scientific	Cat# A14527; RRID: CVCL_D615
Software and algorithms
FlowJo	BD Biosciences	N/A
Prism 9	GraphPad	N/A