FIG. 2.

Accumulation of SulA2 in cells overproducing ClpYQ. Cells transformed with either pACYC184 (lanes 2 and 3) or pWF1 (clpQ⁺ clpY⁺), pWF2 (clpQ::cat clpY⁺), or pWF3 (clpQ⁺ clpY::cat) were grown at 30°C in LB to an optical density at 595 nm of 0.5 and induced with IPTG at a final concentration of 5 mM for 30 min. Samples were subjected to electrophoresis and Western blotting for SulA as described in Materials and Methods. The lon⁺ host was SG22163, and the Δlon host was SG22186; both of these strains carry lacI^q integrated at the mal locus and the plac::SulA2 plasmid. Lane 1 was a control loaded with purified SulA.