TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype | Reference or source |
|---|---|---|
| Strains | ||
| DB1255 | recBC sbcB15 hsdR supF8 | 53 |
| JK6214 | Δ(CP54) (SsrA−) Δlon-510 cpsB10::lac | 27 |
| JM101 | Δ(lac-proAB) supE thi (F′ traD36 proAB lacIqlacZΔM15) | 36 |
| OHP3 | Δlon-510 leu::Tn10 ftsZ(SfiB*) | 8 |
| SG1185 | clpY::cat recBC sbcB15 hsdR supF8 | DB1255 + pWF3 (linearized) |
| SG1186 | clpQ::cat recBC sbcB15 hsdR supF8 | DB1255 + pWF2 (linearized) |
| SG12064 | clpQ::cat | C600 + P1 (SG22192) |
| SG12065 | clpY::cat | C600 + P1 (SG22193) |
| SG20780 | Δlon-510 cpsB10::lac-Mu-λ imm | 3 |
| SG20781 | lon+ cpsB10::lac-Mu-λ imm | 3 |
| SG22119 | Δlac lon+ clp+ | Equivalent of SG20250 (15) |
| SG22163 | malP::lacIq | 14 |
| SG22186 | malP::lacIq Δlon rcsA::kan | 14 |
| SG22192 | clpQ::cat | SG22119 + P1 (SG1186) |
| SG22193 | clpY::cat | SG22119 + P1 (SG1185) |
| SG22205 | malP::lacIqclpQ::cat | SG22163 + P1 (SG22192) |
| SG22206 | malP::lacIqclpY::cat | SG22163 + P1 (SG22193) |
| SG22207 | malP::lacIq Δlon rcsA::kan clpQ::cat | SG22186 + P1 (SG22192) |
| SG22208 | malP::lacIq Δlon rcsA::kan clpY::cat | SG22186 + P1 (SG22193) |
| SG22224 | malP::lacIq Δlon rcsA::kan ftsZ(SfiB*) leu::Tn10 | SG22186 + P1 (OHP3) |
| SG22225 | malP::lacIq Δlon rcsA::kan clpQ::cat ftsZ(SfiB*) leu::Tn10 | SG22224 + P1 (SG12064) |
| SG22226 | malP::lacIq Δlon rcsA::kan clpY::cat ftsZ(SfiB*) leu::Tn10 | SG22224 + P1 (SG12065) |
| Plasmids | ||
| pWF1 | clpQ+ clpY+ tetr (pACYC184) | pACYC184 (EcoRI-ScaI) + PCR fragment from pWPC80 |
| pWF2 | clpQ::cat clpY+ tetr | NsiI-cut pWF1 + cat |
| pWF3 | clpQ+ clpY::cat tetr | PstI-cut pWF1 + cat |
| pWF4 | Δ(clpQ-clpY)::cat | NsiI-PstI-cut pWF1 + cat |
| plac::SulA2 | plac-sulA2 Ampr | Derivative of p21 (49) |
| pWPC80 | clpQ+ clpY+ tetr | pACYC184 + EcoRI fragment from Kohara phage 4H12 |
| pUC4K | pUC4 kanr | 52 |
| pCAT19 | pUC19 cat | 9 |