TABLE 3.
ClpY and ClpQ contribute to the MMS sensitivity of lon mutants
| Straina | Genotype | EOP
on MMSb at a temp (°C) of:
|
|||
|---|---|---|---|---|---|
| 32 | 37 | 39 | 41 | ||
| SG22163 | Wild type | 1 | 1 | 1 | 1 |
| SG22186 | Δlon rcsA::kan | 10−5 | 10−3 | 10−2 | 0.03 |
| SG22205 | clpQ::cat | 0.7 | 0.7 | 0.7 | 0.7 |
| SG22206 | clpY::cat | 1.0 | 0.8 | 1.0 | 1.0 |
| SG22207 | Δlon rcsA::kan clpQ::cat | 10−5 | 10−6 | 10−6 | |
| SG22208 | Δlon rcsA::kan clpY::cat | 10−4 | 10−5 | 10−5 | 10−5 |
a
All strains carry lacIq on the chromosome.
b
Cells were grown at 30°C, diluted into buffer, spotted on LB or LB-MMS plates, and incubated at the indicated temperatures. EOP on LB at 32 and 41.5°C were essentially identical.