TABLE 1.

Nucleotide and derived amino acid sequences of the regions corresponding to periplasmic turns and surface-exposed loops

OmpA turn and loop variants (reference)	DNA and derived amino acid sequencesa	Relevant restriction site(s)
Periplasmic turns
T2, wild type	Gly Tyr Pro Ile Thr	BstEII
	84  85  86  87  88
	GGT TAC CCA ATC ACT
T2H1 (36)	Gly Tyr Pro Lys Leu Gly Thr	HindIII
	84  85  86               88
	GGT TAC CCC AAG CTT GGG ACT
T2M1 (this study)	Gly Tyr Pro Lys Leu Arg Arg Thr Arg Ala Ser Gln Leu Gly Thr	BsmBI, MluI, BsmBI
	84  85  86                                              88
	GGT TAC CCC AAG CTG AGA CGC ACG CGT GCG TCT CAG CTT GGG ACT
T2S0 (this study)	Gly Tyr Pro Gly Thr	SmaI
	84  85  86      88
	GGT TAC CCC GGG ACT
T3, wild type	Ala Ile Thr	None
	130 131 132
	GCG ATC ACT
T3B0 (36)	Val Ile Thr	BclI
	131 132
	GTG ATC ACT
T3S4 (36)	Val Ile Arg Arg Arg Val Asp Ala Ser Thr Arg Arg Arg Ile Thr	SalI (4 times)
	131                                                 132
	GTG ATC CGT CGA CGC GTC GAC GCG TCG ACG CGT CGA CGG ATC ACT
T3S1 (this study)	Val Ile Arg Arg Arg Ile Thr	SalI
	131                 132
	GTG ATC CGT CGA CGG ATC ACT
T3B1 (this study)	Val Ile Arg Arg Ala Gly Cys Ala Gln Pro Ala Arg Arg Ile Thr	BspMI, BssHII, BspMI
	131                                                 132
	GTG ATC GCT CGA GCA GGT TGC GCG CAA CCT GCT CGA CGG ATC ACT
Surface-exposed loops
ΔL1 (this study)	Tyr Ser Arg Glu Asn	XbaI
	18          32  33
	TAC TCT AGA GAA AAC
ΔL2 (this study)	Met Pro Arg Lys Ala	AvrII
	61  62      73  74
	ATG CCT AGG AAA GCT
ΔL3 (this study)	Ala Asp Thr Ser Val	SpeI
	104 105 106     119
	GCA GAC ACT AGT GTT
ΔL4 (this study)	Asn Ala Ser Asp Asn	NheI
	146         158 159
	AAC GCT AGC GAC AAC

^aIntroduced linker sequences of turn variants and introduced nucleotides of loop deletion mutants are shown in boldface; relevant restriction sites are underlined. Wild-type amino acid residues are numbered according to the mature wild-type protein.