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Specific activities of E. chrysanthemi 3937 endopectate lyases at their pH optima with polygalacturonate as the substratea
| Enzyme | pH | Sp act (U · mg−1) |
|---|---|---|
| PelA | 8.0 | 36 |
| PelB | 9.0 | 532 |
| PelD | 8.0 | 467 |
| PelI | 9.0 | 104 |
| PelL | 8.0 | 1.9 |
Conditions of this experiment included the following: 2.5 g of polygalacturonate liter−1, 1 mM CaCl2, and 20 mM Tris-HCl (pH 8.0) or 20 mM 2-amino-methyl-propanol–HCl (pH 9.0) at 37°C.
