TABLE 3.
Effects of DcuC and DcuB inactivation on exchange and uptake activities in strains with various dcu gene compositions
| Activity measured | Strain (genotype) | Carriers present | Transport activity (μmol/min/mg dry wt) | Change in activity (fold) with inactive
|
|
|---|---|---|---|---|---|
| DcuCa | DcuBb | ||||
| Exchange [14C]fumarate-succinate | AN387 (parental) | DcuA DcuB DcuC | 21.8 | 1.8 | 0.49 |
| JRG2821 (dcuA) | DcuB DcuC | 29.5 | 1.4 | 0.18 | |
| JRG2813 (dcuB) | DcuA DcuC | 10.6 | 1.5 | NAc | |
| IMW157 (dcuC) | DcuA DcuB | 39.7 | NA | 0.47 | |
| Uptake of [14C]fumarate | AN387 (parental) | DcuA DcuB DcuC | 13.9 | 1.2 | 0.39 |
| JRG2821 (dcuA) | DcuB DcuC | 11.6 | 1.4 | 0.16 | |
| JRG2813 (dcuB) | DcuA DcuC | 5.4 | 1.5 | NA | |
| IMW157 (dcuC) | DcuA DcuB | 16.7 | NA | 0.47 | |
a
Same as the reference strain but dcuC::mini-Tn10.
b
Same as the reference strain but dcuB::Kanr.
c
NA, not applicable.