FIG. 2.

Construction of strain M-9. (A) Disruption of csmA by pMΔA15. The 4.2-kb PstI-SphI DNA fragment from pMΔA15 was used for transformation of A. nidulans ABPU1. The thin line at the top represents the probe used for Southern analysis. Representative restriction sites are abbreviated as follows: B, BamHI; E, EheI; H, HindIII; X, XhoI. (B) Southern analysis of HindIII- and XhoI-digested total DNA of strain M-9 probed with the 6.6-kb EheI-KpnI fragment from pMK10 (10). Band shifts from 3.6 and 4.7 kb (lane 1; ABPU1) to 2.4 and 4.2 kb (lane 2; M-9), respectively, were confirmed.