Constructions of strains CH5 (alc-chs type) (A) and CS4
(alc-csm type) (B). The 6.7-kb SpeI-NaeI fragment
from pM-ALC-CHS5 (A) and PstI-digested pM-ALC (B) were used
to transform A. nidulans ABPU1. The alcA promoter
is shown as a hatched box. argB is shown as an open box. The
small bar indicates the probe used for Northern analysis.
Representative restriction site abbreviations: H,
HindIII; V, EcoRV; P, PstI; N,
NaeI; S, SpeI.