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Northern analysis of total RNAs from CH5, CS4, and M2-6. CH5 (lanes 1 and 2), CS4 (lanes 3 and 4), and M2-6 (lanes 5 and 6) were grown in MMT medium (lanes 1, 3, and 5) for 5 days or in MM medium (lanes 2, 4, and 6) for 3 days. RNA was isolated as described in Materials and Methods. Approximately 3 μg of RNA was loaded on each lane. The 0.4-kb EcoRV fragment of pM-ALK-CHS was used as a probe. The positions of rRNAs are indicated by the bars on the left.
