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. 2022 Mar 2;94(10):4141–4145. doi: 10.1021/acs.analchem.1c05614

Figure 2.

Figure 2

LAP-MALDI MS analysis of an angiotensin-converting enzyme assay with alternating enzyme-treated and untreated samples for Angiotensin I (Ang I, first row) and N-Hippuryl-His-Leu hydrate (HHL, second row). a) From top to bottom: XIC of [HHL+H]+ (m/z 430.21, substrate), [Ang I + 2H]2+ (m/z 648.84, substrate), His-Leu (m/z 269.15, product), and Angiotensin II [Ang II + H]+ (m/z 1046.54, product). b) Overlay of chromatograms shown in a. Average data acquisition speed was 16 samples/s using a stage speed of 100 mm/s.