Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 17;8(33):eabp9457. doi: 10.1126/sciadv.abp9457

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).

This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 8. — (A and B) Helicase assays in the presence (+) of p44 and Eg5 and increasing amounts of XPD/WT, XPD/T425A, and XPD/T425D. Single- and double-stranded DNA are indicated. (C) XPD/WT and XPD/R683W cells overexpressing XPD/WT, XPD/T425A, or XPD/T425D were treated with increasing doses of UV-C, and cell survival was determined 48 hours later. Data were normalized to unexposed cells (means ± SD of two experiments in triplicates). Significant statistical difference (Student’s t test): between XPD/WT and XPD/R683W cells at 5 J/cm² (P < 0.001) and at 10, 20, and 30 J/cm² (P < 0.0001); between XPD/R683W and XPD/R683W + XPD/WT or XPD/R683W + XPD/T425A cells at 10, 20, and 30 J/cm² (P < 0.0001); and between XPD/R683W and XPD/R683W + XPD/T425D cells at 5 (P < 0.01), 10, and 20 J/cm² (P < 0.0001) and at 30 J/cm² (P < 0.001). (D) XPD/R683W cells overexpressing XPD/WT, Eg5/WT, Eg5/S1033A, or Eg5/S1033E were treated as indicated (C) (means ± SD of two experiments in triplicates). Significant statistical difference (Student’s t test) between XPD/R683W + XPD/WT cells and XPD/R683W, XPD/R683W + Eg5/WT, XPD/R683W + Eg5/S1033A, or XPD/R683W + Eg5/S1033E at 10, 20 (P < 0.0001), and 30 J/cm² (P < 0.0001). (E) In vitro transcription assay with AdMLP template, RNAPII, TFIIA, TFIIB, TFIID(TBP), TFIIE, TFIIF core-IIH, CAK and increasing amounts of XPD/WT, XPD/T425A, or XPD/T425D. (F) Transcription assay with increasing amounts of Eg5. (G) RARβ2 gene expression (normalized to the GAPDH RNA amount) after 8 hours of t-RA treatment in XPD/WT and XPD/R683W cells overexpressing XPD/WT, XPD/T425A, or XPD/T425D. The RARβ2 mRNA expression is presented as n-fold induction relative to nontreated cells (means ± SD of two experiments in triplicates; ***P < 0.001, Student’s t test). (H) RARβ2 gene expression in XPD/R683W cells overexpressing either Eg5/WT, Eg5/S1033A, or Eg5/S1033E (means ± SD of two experiments in triplicates).