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. 1999 Jun;181(12):3743–3750. doi: 10.1128/jb.181.12.3743-3750.1999

FIG. 2.

FIG. 2

FIG. 2

Identification of the fla operon promoter. (A) DNA sequence including the proposed −10 and −35 regions of Pfla. The large arrow indicates the start site of transcription determined by primer extension. M indicates the first amino acid of the Tap1 polypeptide. (B) Primer extension assay to determine the start site of transcription of Pfla. A, C, G, and T indicate nucleotides used to generate a size ladder with unrelated DNA. Lanes 1 and 2 contain the primer extension reaction products of 2 and 5 μl, respectively.