Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HEK293S | Expression Systems, courtesy of Dr Ai-Wu Zhou (Shanghai Jiao Tong University School of Medicine) |
RRID:CVCL_A784 | |
| Cell line (Spodoptera frugiperda) | Sf9 | Expression Systems, courtesy of Dr Ai-Wu Zhou (Shanghai Jiao Tong University School of Medicine) |
RRID:CVCL_0549 | |
| Antibody | Anti-mouse CD45.2 Alexa Fluor 700 (Mouse monoclonal) | BD | Cat# 560693 RRID:AB_1727491 |
FACS (1:200) |
| Antibody | Anti-mouse CD11b APC (Rat monoclonal) | eBioscience | Cat# 17-0112-82 RRID:AB_469343 |
FACS (1:500) |
| Antibody | Recombinant Anti-Human IgG antibody (Rabbit monoclona) | Abcam | Cat# ab109489, RRID:AB_10863040 | IF (1:150) |
| Antibody | Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor 488 (Donkey polyclonal secondary) | Thermo Fisher Scientific | Cat# A-21206, RRID:AB_2535792 | IF (1:400) |
| Antibody | Anti-Lambda Light Chain Monoclonal Antibody, Biotin (Mouse monoclonal) | BD Biosciences | Cat# 555794, RRID:AB_396129 | ELISA (1:2000) |
| Antibody | Anti-Dsg1 TTP1-8-15(IgG1) Biotin (human monoclonal) | This paper | ELISA (1 μg/ml) | |
| Antibody | Anti-Human IgG-Fc Fragment Antibody HRP (Goat polyclonal) | Bethyl | Cat# A80-104P, RRID:AB_67064 | ELISA (1:10,000) |
| Antibody | Anti-Human IgG1 Hinge-HRP (Mouse monoclonal) | SouthernBiotech | Cat# 9052–05, RRID:AB_2796619 | ELISA (1:3160) |
| Antibody | Anti-Human IgG4 Fc-HRP (Mouse monoclonal) | SouthernBiotech | Cat# 9200–05, RRID:AB_2796691 | ELISA (1:3160) |
| Antibody | Anti-Human IgG1 Fc Secondary Antibody, HRP (Mouse monoclonal) | Thermo Fisher Scientific | Cat# MH1715, RRID:AB_10374315 | ELISA (1:2000) |
| Antibody | Anti-Human IgG4 Fc Secondary Antibody, HRP (Mouse monoclonal) | Thermo Fisher Scientific | Cat# MH1742, RRID:AB_2539714 | ELISA (1:2000) |
| Antibody | Anti-Human IgG (H+L) (Goat polyclonal) | Jackson ImmunoResearch Labs | Cat# 109-005-088, RRID:AB_2337539 | ELISA (2 μg/ml) |
| Antibody | Anti-Human IgG-heavy and light chain Antibody HRP (Goat polyclonal) | Bethyl | Cat# A80-219P, RRID:AB_67076 | ELISA (1:10,000) |
| Peptide, recombinant protein | Streptavidin-HRP | BD Biosciences | Cat# 554066, RRID:AB_2868972 | ELISA (1:1000) |
| Peptide, recombinant protein | Recombinant Human ADAMTS13 (Full Length) Protein, CF | R&D Systems | Cat# 6156-AD | ELISA (2 μg/ml) |
| Commercial assay or kit | Human ADAMTS13 Quantikine ELISA Kitg | R&D Systems | Cat# DADT130 | |
| Chemical compound, drug | FRETS-VWF73 | Anaspec | Cat# 63728–01 | |
| Chemical compound, drug | Dispase II | Roche | Cat# 4942078001 | |
| Chemical compound, drug | DNase I | Sigma | Cat# D5025 | |
| Chemical compound, drug | Collagenase IV | Sigma/biosharp | Cat# C5138 | |
| Software, algorithm | GraphPad Prism | GraphPad Prism | RRID:SCR_002798 | |
| Other | DAPI stain | Invitrogen | DD3571 | (0.5 µg/ml) |