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. 2022 Feb 8;122(7):1147–1158. doi: 10.1055/a-1723-1880

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The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. ( https://creativecommons.org/licenses/by-nc-nd/4.0/ )

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.

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Fig. 6 — Thrombus formation in whole blood. ( A ) Thrombus formation upon treatment with 600 ng/mL pneumolysin (Ply) or pneumolysin preincubated with IVIG or trimodulin. A quantitative assessment of thrombus formation was performed by analyzing the area of the collagen-coated microchannel slide that was covered by thrombi. Curves represent the mean of platelets of three donors. As a control, whole blood was incubated with PBS only. ( B ) Thrombus formation upon treatment with immunoglobulin preparations (without pneumolysin) to investigate their effects alone on the thrombus formation. A quantitative assessment of thrombus formation was performed by analyzing the area covered. Curves represent the mean of platelets of three donors. As a control, whole blood was incubated with PBS only. IVIG, intravenous immunoglobulin; PBS, phosphate-buffered saline.