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Effect of overproduction of NifY on nitrogenase activity in the wild type and a nifL mutant. Strains were derepressed for nif function in the presence of IPTG at the concentrations shown for 4 h. Nitrogenase activity was measured as described in Materials and Methods for strains overexpressing nifY in the UN5397 (nif+; □, solid line) and UN5406 (nifL; ○, dotted line) backgrounds.
