Figure 3.

Treatment with RU.521 abrogated viability of TNBC cells cultured in serum-depleted media. (A), MDA-MB-231 and SUM159 cells were cultured in normal growth media containing 10% FBS or serum-depleted media for 48 hr. Bright field images were taken at 400x magnification. White arrowheads indicate the cytoplasmic vacuoles. Bar, 25 μm. (B–E) MDA-MB-231 (B, C) and SUM159 (D, E) cells cultured in normal growth media containing 10% FBS (blue) or serum-depleted media (green) treated with different doses (B, D) and at the dose of 10 μM (C, E) of RU.521 for 48 hr. Cell viability was determined by crystal violet staining and detected by absorbance at 595 nm. The results of three independent experiments are shown as means ± SD. Statistical significance was calculated by Student’s t-test. ***, p < 0.001. The half maximal inhibitory concentration (IC₅₀) of RU.521 for each cell line is indicated.