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In vivo complementation chromosomal temperature-sensitive dnaG by plasmid-expressed K241R mutant primase. E. coli KY1378 containing temperature-sensitive dnaG2903 gene was transformed with pGNG7 (wild-type primase), pGNG7-K241R (mutant primase), and the control pET-21d vector (no insert). The transformed cells were plated on LB plates containing 100 μg of ampicillin per ml and incubated at 30 or 42°C overnight.
