FIG. 1.
Identification of the rsd promoters. (A) Primer extension analysis was carried out for RNA extracted from the exponentially growing cells (Log lane) and the stationary-phase cells (Stationary lane). (B) Primer extension products were analyzed by electrophoresis on a denatured polyacrylamide gel together with sequence ladders. P1, transcript from rsdP1 promoter; P2, transcript from rsdP2 promoter. (C) Primer extension analysis was carried out for RNA extracted from stationary-phase cells of wild-type (WT) strain ZK126 (rpoS+) and mutant strain ZK1000 (rpoS). (D) In vitro transcription of the rsd promoters by Eς70 or EςS holoenzymes. Transcription products were analyzed by polyacrylamide gel electrophoresis in the presence of 8 M urea. (E) Nucleotide sequence of the upstream region of rsd. (F) Primers used in this study. (G) Sequence comparison of rsdP2 with the known gearbox promoters.