FIG. 3.
Effect of the rsd mutation on bolAP1-directed transcription. Cells were grown in LB medium. Growth (circles) was monitored by measuring turbidity, while β-galactosidase activity (triangles) was determined at the indicated times. Aliquots containing the same cell numbers were subjected to a quantitative Western blot assay for measurement of ςS levels. (A) Strain MJ31 [MC4100 λ(bolA-lacZ); open symbols] and its isogenic rsd mutant, MJ35 [MC4100 rss::Km λ(bolA-lacZ); solid symbols]. (B) MJ31 [MC4100 λ(bolA-lacZ)] carrying either pACYC184 (open symbols) or pACYCRsd (solid symbols). (C) MJ35 [MC4100 rsd::Km λ(bolA-lacZ)] carrying either pACYC184 (open symbols) or pACYCRsd (solid symbols). (D) MJ35 [MC4100 rsd::Km λ(bolA-lacZ)] carrying either pBAD22A (open symbols) or pBF1 (solid symbols). Arabinose was added at 2 h of culture (time point 1).