FIG. 5.

Effect of Rsd production on ompF-directed transcription. Cells of MH513 [MH20 λ(ompF-lacZ)] carrying either pBAD22A (open symbols) or pBADRsd (closed symbols) were grown in LB medium. Growth (circles) was monitored by measuring turbidity, while β-galactosidase activity (triangles) was determined at the indicated times. Overproduction of Rsd was induced by adding arabinose (0.02%) at the early exponential phase (A), at the middle of the exponential phase (B), and after entry into the stationary phase (C). Aliquots containing the same cell numbers were subjected to quantitative Western blotting for measurement of the Rsd level.