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. 2022 Aug 8;83:104204. doi: 10.1016/j.ebiom.2022.104204

Figure 2.

Figure 2

Medrysone promotes oligodendrocyte recovery and remyelination at the corpus callosum junction. Representative pictures of the corpus callosum junction (CCJ, Figure 1c) stained for MBP, GSTπ, Caspr (a-d, a’-d’) Olig2 and CC1 (g-l), Sox10 and Bcas1 (m-r) protein expression. (s) Quantitative analysis of MBP stained structures revealed a decrease of myelin expression in the demyelination group with saline treatment leading to weak remyelination and medrysone significantly promoting MBP recovery at the early time point (1 WPC). (t, u, v) Similarly, the numbers of GSTπ positive, Olig2/CC1- and Sox10/Bcas1 double-positive cells recovered upon medrysone application accompanied by improved generation of nodes of Ranvier (w). (x) Oligodendrocyte maturation and representative markers (x’) graphical and in vivo representation of nodes of Ranvier immunofluorescence (Caspr+/MBP+). Bars in (s) correspond to mean fluorescence intensity ± standard error of the mean (SEM), bars (t, u, v) correspond to mean cell numbers per mm2 ± standard error of the mean (SEM), bars in (w) correspond to mean number of nodes ± standard error of the mean (SEM). Statistical significance was calculated using a two-way ANOVA with multiple comparison Bonferroni post-test (*p < 0.05, **p < 0.01, and ***p < 0.001). Number of animals per analysis: n=6 (s, t); n=5 (u, v, w). Exact p-values according to sequence from left to right: s: (p= 0.000000383874685, p=0.000000935915998, p=0.000001608060795, p=0.000000637203217, p=0.000000164768189, p=0.000004042884687, p=0.000001570954253, p=0.000000394110251). t: (p=0.001937648865078, p=0.003177866419346). u: p=0.045380409230564, p=0.012638408327068, p=0.00011622977178, p=0.036252296508041, p=0.000318271010733, p=0.011713553263055). v: (p=0.023166172011608, p=0.04065251019751, p=0.000116654567343, p=0.000261656183587, p=0.001371706447911). w: (p=0.000125063209522, p=0.000773952216255, p=0.018530905333733, p=0.000384534736556, p=0.001076299627656, p=0.002472602001283, p=0.007086659102636).