Figure 3.

Quantification of hGFAP-GFP positive cells in the corpus callosum junction and dorsal SVZ. (a-m) Representative pictures of GFP-positive cells at the CCJ, a-CC and the d-SVZ with cell nuclei visualized using Dapi. (g) Quantification of GFP-positive cells revealed similar number of GFP expressing cells in all groups at the corpus callosum junction. (n) In the a-CC demyelination induced the degree of GFP-positive cells (versus control) and a mild induction between medrysone and saline groups was found at 3 WPC. Moreover, in the d-SVZ (o), GFP-positive cells were enriched upon medrysone treatment at 1 WPC (versus control), sharply dropping thereafter (3 WPC). Abbreviation: non-significant (ns). Bars correspond to mean cell numbers per mm² ± standard error of the mean (SEM). Statistical significance was calculated using Kruskal-Wallis test with Dunn's post-test (g) and two-way ANOVA with multiple comparisons Bonferroni post-test (n; o; *p < 0.05, **p < 0.01, and ***p < 0.001). Number of animals n=6 (g, o), n=7 (n). Exact p-values according to sequence from left to right: n: (p=0.000388398742168; p=0.000104914644292, p=0.000001511008705, p=0.000077138716235, p=0.019669035728742, p=0.028226088991489). o: (p=0.006937732126289, p=0.005565374980289, p=0.000255068999518).