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. 2022 Aug 5;45(8):550–563. doi: 10.14348/molcells.2022.0009

Fig. 3. SETD5 KD induces Huh7 and HepG2 cell apoptosis.

Fig. 3

(A and B) Left: Effects of SETD5 KD in Huh7 and HepG2 cells on the cell cycle were examined by FACS analysis to detect propidium iodide signals. Right: Quantification of cell cycle distribution as determined using a flow cytometer. Values are the mean ± SEM of biological triplicate experiments. (C and D) Left: Effects of SETD5 KD in Huh7 and HepG2 cells on cell death were examined by FACS analysis based on annexin V and propidium iodide double staining. Right: Quantification of the dead cell population as determined using a flow cytometer. Values are the mean ± SEM of biological triplicate experiments (n = 3, *P < 0.05, one-way ANOVA). (E) Cleaved PARP in Huh7 and HepG2 cells was examined by immunoblotting with anti-PARP antibody. The intensities of immunoblotting signals were semiquantitatively measured by ImageJ.