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. 2022 Aug 17;12:13953. doi: 10.1038/s41598-022-17474-8

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

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(A) Schematic of lcrV RNA of different lengths and the complementary region associated with crRNA set 0056. (B) Comparison of fluorescence sums normalized to internal control between different target lengths (Target A and Target B, as defined in panel A), where bars represent mean and error bars represent standard deviation (n = 3). (C) Comparison of normalized fluorescence sums from different target regions, where values represent mean, dot size represents relative standard deviation (n = 3), red labels represent crRNAs bearing unimproved activity (p-value < 0.5 as determined by analysis of variance and Tukey test against internal control) against both target lengths, and black labels represent Cas13a activation from crRNA that improved to within 50% of internal control after shortening target length.