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. 2022 Aug 17;13:4848. doi: 10.1038/s41467-022-32515-6

Fig. 6. Rapid mobilization of lysosomal proteolytic capacity in response to mTORC1 inactivation.

Fig. 6

a Degradation of lysosomally preloaded DQ BSA in MEFs upon torin 1 treatment [400 nM]. Scale bars = 20 µm. b Quantification of DQ BSA in Lamp1-containing lysosomes of cells shown in a. Data are mean ± SD (25 fields of view). c Lysosomal degradation of magic red substrates of cathepsins B and L in MEFs after 1 h pretreatment ± torin 1 [400 nM]. Scale bars = 20 µm. d Fraction of lysosomes with high cathepsin B/L activity in cells treated as in c. Data are replicate mean ± SEM (closed circles) and fields of view (open circles; ≥10 per replicate). e Degradation of lysosomally preloaded DQ BSA in MEFs after 0–2 h torin 1 [400 nM]. Scale bars = 10 µm. f Magnification of areas highlighted in e. g Fraction of lysosomes with high DQ BSA degradation in cells treated as in e. Data are replicate mean ± SEM (closed circles) and fields of view (open circles; ≥10 per replicate). b One representative of n = 3 biologically independent experiments; d n = 4, g n = 3 biologically independent experiments. p-values were calculated using a two-tailed unpaired t-test with Welch correction. Source data are provided as a Source data file.