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. 2022 Aug 17;12:13927. doi: 10.1038/s41598-022-17495-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Antimicrobial efficacy and cell viability under continuous exposure to differing levels of ozone (2–8 mg/h). Antimicrobial effect against (a) P. aeruginosa and (b) E. coli bacteria cultures in PBS over the course of 8 h. (c) Cell viability of human fibroblasts treated with 6 h of ozone at 2–8 mg/h at 37 °C. Viability was measured 1 day, 3 days, and 7 days after treatment ended. (d) Live/Dead staining of human fibroblast cells exposed to varying levels of ozone therapy 1 day and 7 days after treatment. Error bars denote standard deviation.