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. 2022 Aug 8;3(3):101607. doi: 10.1016/j.xpro.2022.101607

Figure 2.

Figure 2

Induction of spontaneous brain tumors via injection of plasmid DNA into the brain

(A) Hypothermal anesthesia setup for pups.

(B) Plasmid DNA solution mixed with Fast Green.

(C) The original (a) and the tip-removed (b) micropipettes.

(D) A micropipette connected with a silicon tube.

(E) A micropipette tip loaded with the plasmid DNA solution.

(F) Injection point at approximately 1/3 the length of the virtual line between the right eye and lambda.

(G) Confirmation of plasmid DNA injection into the lateral ventricle via lightening brain with lamp.

(H) A Tweezer electrode.

(I) Transfecting plasmid DNA into the brain via electroporation.

(J) Confocal microscopy (red: tdTomato+ tumors; DAPI: nuclei) of brain tumor tissue sections from the spontaneous brain tumor model in mice with a hunched posture (20 weeks). Zoomed image of the border of the tumor tissue is shown on the right. Scale bar = 1000 μm (left) and 100 μm (right).