Enzyme mixture
| Reagent | Final concentration | Volume |
|---|---|---|
| Collagenase IV stock | 2 mg/mL | 0.5 mL |
| DNase I stock | 60 μg/mL | 0.15 mL |
| Isolation buffer | N/A | 4.35 mL |
| Total | N/A | 5 mL |
Prepare the day of experiment and keep at RT.
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