Table 5.

Prediction of EGFR mutations and activity.

No	Mutant	PolyPhen-2 HumDiv		PolyPhen HumVar		Provean		SIFT		FATHMM					PANTHER
		Score	Prediction	Score	Prediction	Score	Prediction	Score	Prediction	Domain Involved	Cancer		Inherited disease
											Score	Prediction	Score	Prediction	Preservation time	Message	Pdel
1	A289D	0.996	PD	0.993	PD	−5.24	Del	0.001	D	Growth factor receptor domain	−1.01	Cancer	−0.01	T	797	PD	0.74
2	A289T	0.987	PD	0.974	PD	−3.54	Del	0.001	D	Growth factor receptor domain	−1.02	Cancer	−0.02	T	797	PD	0.74
3	A289V	0.997	PD	0.989	PD	−3.56	Del	0.001	D	Growth factor receptor domain	−1.04	Cancer	−0.04	T	797	PD	0.74
4	C620W	1	PD	0.998	PD	−10.32	Del	0	D	Growth factor receptor domain	−3.32	Cancer	0.00	T	911	PD	0.85
5	C620Y	1	PD	0.989	PD	−10.32	Del	0	D	Growth factor receptor domain	−3.3	Cancer	0.02	T	911	PD	0.85
6	E709K	0.974	PD	0.721	Pos D	−3.38	Del	0.003	D	Protein kinase−like (PK−like)	−1.93	Cancer	0.07	T	842	PD	0.78
7	G598V	0.997	PD	0.849	Pos D	−8.43	Del	0.004	D	Growth factor receptor domain	−2.26	Cancer	1.06	T	797	PD	0.74
8	H304Y	0	benign	0.005	benign	−2.01	Neut	1	T	Growth factor receptor domain	−1.02	Cancer	−0.02	T	456	PD	0.57
9	L62R	0.795	Pos D	0.553	Pos D	−2.02	Neut	0.006	D	Receptor L domain	−0.6	Passenger/Other	−1.27	T	455	PD	0.57
10	P596R	1	PD	0.999	PD	−8.44	Del	0	D	Growth factor receptor domain IV	−3.96	Cancer	−0.38	T	911	PD	0.85
11	P596S	1	PD	0.968	PD	−7.5	Del	0.005	D	Growth factor receptor domain IV	−3.93	Cancer	−0.35	T	911	PD	0.85
12	R108G	1	PD	1	PD	−5.87	Del	0.01	D	Receptor L domain	−0.81	Cancer	−1.48	T	842	PD	0.78
13	R108K	1	PD	1	PD	−2.59	Del	0.001	D	Receptor L domain	−0.78	Cancer	−1.44	T	842	PD	0.78
14	R222C	1	PD	1	PD	−6.52	Del	0	D	Growth factor receptor domain	−1.05	Cancer	−0.05	T	797	PD	0.74
15	R252C	1	PD	0.993	PD	−3.25	Del	0.025	D	Furin−like cysteine rich region	−2.74	Cancer	−2.08	D	456	PD	0.57
16	R252P	0.998	PD	0.991	PD	−3.17	Del	0.058	T	Furin−like cysteine rich region	−2.73	Cancer	−2.06	D	456	PD	0.57
17	S645C	0.999	PD	0.982	PD	−1.96	Neut	0.187	T	−	−1.07	Cancer	−0.94	T	361	Pos D	0.5
18	T263P	0.952	Pos D	0.913	PD	−1.38	Neut	0.087	T	Furin−like cysteine rich region	−2.51	Cancer	−1.85	D	176	Prob Benign	0.27
19	T363I	1	PD	0.994	PD	−5.07	Del	0.001	D	Receptor L domain	−0.96	Cancer	−1.62	D	842	PD	0.78
20	V774M	1	PD	0.994	PD	−1.61	Neutral	0.001	D	Protein kinase−like (PK−like)	−2.3	Cancer	−0.3	T	456	PD	0.57
21	L861Q	1	PD	0.993	PD	−5.29	Del	0.008	D	Protein tyrosine kinase	−1.88	Cancer	−1.59	D	797	PD	0.74
22	H773_V774dup	NA	NA	NA	NA	−10.34	Del	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA

PD probably damaging, Pos D possibly damaging, Del Deleterious, Neut Neutral, D Damaging, T Tolerated.