Table 2.
Measurements of nuclear DNA content in picograms (pg, mean ± standard deviation of all nuclei) with corresponding C-value for different life phase stages of the brown macroalga Saccharina latissima, obtained by comparison of fluorescence intensity values with those of chicken erythrocytes. In case of zoospores (released and unreleased), n is the number of parental sources; in the case of gametophytes, it is the number of single-gametophyte cultures, and in the case of sporophytes, it is the number of individuals. If we detected cell with higher C-value, although in no large numbers, we expressed it with “ + ” or “ + + ” if more abundant. The average of relative nucleus size (NRS) obtain in this study is given (± standard deviation)
| Life phase | n | n° nuclei | NRS | 1C | 2C | 4C | 8–16C |
|---|---|---|---|---|---|---|---|
| Zoospores (released) | 3 | 387 | 0.044 ± 0.01 | 0.76 ± 0.07 | + | - | - |
| Zoospores (unreleased) | 1 | 64 | - | 0.79 ± 0.05 | - | - | - |
| Male gametophytes | 6 | 1464 | 0.043 ± 0.01 | - | 2.01 ± 0.22 | + | - |
| Female gametophytes | 5 | 946 | 0.084 ± 0.03 | - | 2.27 ± 0.56 | + + | + |
| Sporophytes: embryonic | 25 | 408 | 0.095 ± 0.02 | - | 1.64 ± 0.21 | + | - |
| Sporophytes: meristoderm | 7 | 905 | 0.129 ± 0.07 | - | 1.51 ± 0.16 | - | - |
| Sporophytes: cortex/medulla | 7 | 629 | - | - | 2.40 ± 0.41 | + + + | + + + |