Fig. 3.

Notable observations in three Mougeotia strains. Nomarski interference contrast micrographs of the strains Mougeotia sp. SAG 650-1, Mougeotia scalaris SAG 164.80, and Mougeotia sp. MZCH 240; the latter was grown in liquid medium, on solid agar plates, and on agar plates and subjected to 24h of submergence in liquid medium (“shift”). The two SAG strains 650-1 and 164.80 were grown either in liquid or on solid MiEB12 Medium, as indicated by the “L” (liquid medium) or “S” (solid medium) on the bottom left side of the pictures. Notable phenotypic observations include: (a) darkly colored inclusions (sometimes co-occurring with high density of intracellular bodies being trafficked); (b) rhizoid formation in liquid culture; (c) formation of granules, possibly for storage. Also note the twisting chloroplasts, including “edge-on” orientations as a sign for functional chloroplast movement induced by microscope illumination. Labels in the bottom left corner denote: L=liquid-grown, S=solid-grown (agar), S➞L=solid-grown and submerged for 24h