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. 2022 Aug 17;13(8):716. doi: 10.1038/s41419-022-05160-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Immunohistological representation of DDp53 in the contralateral control leg (left) and the leg electroporated with T7-tagged DDp53. DDp53 was detected via T7-tag (purple) and was mainly located in the interstitium of the ipsilateral tibialis anterior, while it was absent in the contralateral control leg. Sarcolemma shown via anti-laminin-staining (green). Nuclear staining via DAPI (blue). B Western blot confirmed the successful overexpression of DDp53 via increased T7-Tag protein levels in the ipsilateral compared to the contralateral control leg. Individual data points represent individual animals. Squares represent the leg that was electroporated with DDp53 and circles the contralateral control leg of the same animals. Western blot images show three representative bands from each condition. Both representative pictures stem from the same membrane and exposure time. All western blots were normalized to total protein per lane. Original, unaltered images of all membranes and the gels that they were normalized to can be found in the data supplement. n = 12, **** indicates a p-value of <0.0001.