Interaction of the Am-MC with dsRNAs
(A) Schematic illustration of Am-SP, Am-MC, and Am-MCH+ used in this study. Interaction between Am-MC and dsRNA stabilizes Am-MCH+, resulting in decreased absorbance at 515 nm. (B, C) The absorbance spectra change of Am-MC by different concentrations of 20-bp poly AU (B) or poly GC (C) dsRNAs. (D) Quantification of the absorbance change at 515 nm by 20-bp poly AU or poly GC dsRNAs. (E) The absorbance spectra of Am-MC when poly AU dsRNAs with different lengths were added; 14 μM 100-bp poly AU, 70 μM 20-bp poly AU, and 140 μM 10-bp poly AU dsRNAs were used.