Figure 3.

dsRNA induction as a dynamic marker for the HMA therapy

(A) Schematic of the process for analyzing dsRNA expression in patient bone-marrow aspirates. (B) The total dsRNA expression in bone-marrow aspirates measured by the absorbance change at 515 nm of Am-MC. A total of 10 paired patient (five non-responders and five responders) samples were analyzed. (C) Expression of eight selected ERV RNAs in bone-marrow aspirates from patients prior to receiving HMAs. Each ERV expression was normalized by the median. (D) The induction of eight selected ERV genes after receiving HMAs was analyzed by RT-qPCR. All ERV RNA expressions were normalized to that of GAPDH mRNA. A paired Student’s t test was used to compare Am-MC spectral change before and after the therapy. A non-paired Student’s t test was used to compare the Am-MC spectral change between responder and non-responder groups. ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.