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. 2022 Jul 21;29:370–383. doi: 10.1016/j.omtn.2022.07.014

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Analysis of dsRNA induction by DAC in solid tumor cell lines

(A) Heatmap of cell viability of different cancer cell lines 4 days after the DAC treatment. (B) Percentage absorbance change of 515-nm peak of Am-MC after incubating with 2 μg of RNase T1-treated RNAs from indicated cells. The average of three biological replicates is shown with error bars indicating SD. (C) Expression change of eight selected ERV RNAs measured by RT-qPCR. All ERV RNA expressions were normalized to that of GAPDH mRNA. The average of three biological replicates were shown with error bars denoting SD. (D) The correlation between cell viability and the absorbance change of Am-MC from day 0 to day 5. (E) The correlation between cell viability and the basal Am-MC absorbance at 515 nm.