Figure 6.

Effect of nc886 in preventing DAC-mediated cell death

(A) Expression of nc886 RNA quantified by RT-qPCR in DAC-treated MCF-7 cells. nc886 RNA expressions were normalized to that of vtRNA1-1. (B) The downregulation of nc886 expression after transfecting cells with siRNAs against nc886. (C) The effect of knockdown of nc886 on cell viability with or without DAC treatment. MCF-7 cells 4 days after DMSO (DAC−) or DAC treatment were used. (D) Western blotting of the PKR signaling pathway when nc886 was knocked down with or without DAC treatment. MCF-7 cells 5 days after DMSO (DAC−) or DAC treatment were used for the analysis. siLuc was used as a transfection control (sinc886−), and TUBB was used as a loading control. Quantified data for biological triplicates is shown below. In all plots, the average of three biological replicates is shown with error bars indicating SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.