TABLE 1.
E. coli strains used in this study
| Strain | Relevant genotype | Source, reference, or construction |
|---|---|---|
| TXΔ11b | Δ(purDH-rrnE-metA) | 12 |
| Strains of TXΔ11 background | ||
| TA340 | TXΔ11 polA1 zih::Tn10 | TXΔ11 × P1.AQ8809, with selection for Tcr, PolA− |
| TA405 | TA340 Δ(rrsB-gltT-rrlB)1::kan+ | TA340 × P1.CC164, with selection for Kmr |
| TA406 | TA340 Δ(rrsB-gltT-rrlB)1::kan+zij::pMA101 | TA405 transformed with pMA101; selection for Apr, Sucs |
| TA410 | TA340 Δ(rrsB-gltT-rrlB)101 | Sucr derivative of TA406; Aps Kms |
| TA415 | TA410 Δ(rrsH-ileV-alaV-rrlH)37::cat+ | TA410 × P1.JP37, with selection for Cmr |
| TA418 | TA410 Δ(rrsH-ileV-alaV-rrlH)37::cat+zaf::pMA103 | TA415 transformed with pMA103; selection for Apr, Kmr, Sucs |
| TA420 | TA410 Δ(rrsH-ileV-alaV-rrlH)103 | Sucr derivative of TA418; Aps Kms Cms |
| TA430 | TA420 Δ(rrsA-ileT-alaT-rrlA)1::cat+polA+ | TA420 × P1.CC164, with selection for Cmr, Tcs, PolA+ |
| TA443 | TA420 Δ(rrsG-gltW-rrlG)33::cat+ | TA420 × P1.JP33, with selection for Cmr |
| TA445 | TA420 Δ(rrsG-gltW-rrlG)33::cat+zfg::pNY30 | TA443 transformed with pNY30; selection for Apr, Kmr, Sucs |
| TA447 | TA420 Δ(rrsG-gltW-rrlG)30::lacZ+ | Sucr derivative of TA445; Aps Kms Cms |
| TA472 | TA410 Δ(rrsA-ileT-alaT-rrlA)1::cat+ | TA410 × P1.CC164, with selection for Cmr, Tcr, PolA− |
| TA476 | TA447 polA+ Δ(rrsA-ileT-alaT-rrlA)1::cat+ | TA447 × P1.CC164, with selection for Cmr, Tcs, PolA+ |
| TA480 | TA410 Δ(rrsA-ileT-alaT-rrlA)1::cat+zih::pNY34 | TA472 transformed with pNY34; selection for Apr, Kmr, Sucs |
| TA485 | TA447 polA+ Δ(rrsA-ileT-alaT-rrlA)1::cat+zih::pNY34 | TA476 × P1.TA480, with selection for Cmr, Kmr, Apr, Tcs, PolA+, Sucs |
| TA488 | TA447 polA+ Δ(rrsA-ileT-alaT-rrlA)34 | Sucr derivative of TA485; Aps Kms Cms |
| TA500 | TA488/pTRNA65 | TA488 transformed with pTRNA65; selection for Spcr |
| TA516 | TA488 Δ(rrsD-ileU-alaU-rrlD)25::cat+/pTRNA65 | TA500 × P1.JP25, with selection for Cmr |
| TA520 | TA488 Δ(rrsD-ileU-alaU-rrlD)25::cat+/pTRNA65 pHK-rrnC+ | TA516 transformed with pHK-rrnC+; selection for Kmr |
| TA520.5 | TA488 Δ(rrsD-ileU-alaU-rrlD)25::cat+/pTRNA66 pHK-rrnC+ | tRNA2Glu deletion from pTRNA65 in TA520, generating pTRNA66 |
| TA525 | TA520.5 Δ(rrsC-gltU-rrlC)15::cat+ilv500::Tn10 | TA520.5 × P1.TA575, with selection for Tcr, rrnC::cat+ |
| TA527 | TA520.5 Δ(rrsC-gltU-rrlC)15::cat+ilv+ | TA525 × P1.JP15, with selection for Ilv+, Tcs |
| TA559.5 | TXΔ11/pBEU49 | TXΔ11 transformed with pBEU49; selection for Apr, Kmr, Tsa |
| TA560c | pur+ rrnE+ metA+ metB1 btuB3191::Tn10/pBEU49 | TA559.5 × Hfr CAG5052, with selection for Tcr Apr, Kmr, Pur+, rrnE+, Met− |
| TA563c | pur+ rrnE+ metA+ metB1 btuB3191::Tn10 | Temperature-resistant derivative of TA560; Aps Kms |
| TA566 | TA563 Δ(rrsA-ileT-alaT-rrlA)1::cat+ | TA563 × P1.CC164, with selection for Cmr |
| TA567 | TXΔ11 Δ(rrsA-ileT-alaT-rrlA)1::cat+ | TXΔ11 × P1.CC164, with selection for Cmr |
| TA568 | TA410 Δ(rrsA-ileT-alaT-rrlA)1::cat+polA+ | TA410 × P1.CC164, with selection for Cmr, Tcs, PolA+ |
| Intermediates in strain constructions | ||
| AQ8809 | polA1 zih::Tn10 | T. Kogoma |
| CAG5052 | KL227 btuB3191::Tn10 | 30 |
| CAG18431 | MG1655 ilv500::Tn10 | 30 |
| CC164 | W1485 Δ(rrsB-gltT-rrlB)1::kan+ Δ(rrsA-ileT-alaT-rrlA)1::cat+ Δ(rrsG-gltW-rrlG)1::spc+ | BAG1 in reference 7 |
| JP15 | W1485 Δ(rrsC-gltU-rrlC)15::cat+ | W1485 ΔC in reference 9 |
| JP25 | W1485 Δ(rrsD-ileU-alaU-rrlD)25::cat+ | W1485 ΔD in reference 9 |
| JP33 | W1485 Δ(rrsG-gltW-rrlG)33::cat+ | W1485 ΔG in reference 9 |
| JP37 | W1485 Δ(rrsH-ileV-alaV-rrlH)37::cat+ | W1485 ΔH in reference 9 |
| TA575 | W1485 Δ(rrsC-gltU-rrlC)15::cat+ilv500::Tn10 | JP15 × P1.CAG18431, with selection for Tcr, Cmr |
a
Ts, temperature sensitive.
b
The remaining genotypes are F− ara Δlac thi.
c
The presence of metA+ metB1 in these strains has not been verified (see Materials and Methods).