Figure 9.

Heat map highlighting universal ligands with high affinities toward four lipocalin and two secretoglobin mammalian respiratory allergens and human LCN1 from dark (high affinity) to faint red (low affinity). Bar on the left represents the number of carbon atoms for each ligand in dark violet < 9, blue 9–12, green 13–16 and yellow > 16. The heat map was created using mean fluorescence inhibition (n = 2) of 5 μM allergen-fluorochrome complexes by 50 μM (100 μM for Fel d 1) of forty-seven competitive ligands.