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Phase contrast microscopy images of cell differentiation culture at day 7 of differentiation. Cells from ISO44 (A) and ISO45 (B) that were growing in SFM were seeded for differentiation at—16 population doublings. After 24 h in SFM, and when the cells reached a 90% confluency, differentiation was induced with DMEM (1 g/L glucose) + 2% FBS.
