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. 2022 Aug 4;16:885775. doi: 10.3389/fnbeh.2022.885775

FIGURE 2.

FIGURE 2

Different startle responses evoked by illumination changes in zebrafish and Dc larvae. (A) Startle responses ± SEM (shaded) of 6 dpf zebrafish AB wildtype (green; n = 60), crystal (blue; n = 60), and Dc (red; n = 40) larvae depicted from 2 s before (2,398 s) to 18 s after (2,418 s) the first light (swimming phase; blue) to dark (dark phase 1; gray) switch (see also Supplementary Figure 1A); a dotted black rectangle indicates the 1 s time interval that was used to compare the velocity of the larvae in (B). (B) Violin plots depicting the velocity of AB, crystal, and Dc larvae during 1 s (2,400–2,401 s) following the first light to dark switch (see also Supplementary Figure 1B). Note that Dc increase their velocity significantly less than AB wildtype and crystal zebrafish. (C) Startle responses of 6 dpf zebrafish AB wildtype (green), crystal (blue), and Dc (red) larvae depicted from 2 s before (2,998 s) to 18 s after (3,018 s) the first dark (gray) to light (yellow) switch (see also Supplementary Figure 1C); a dotted black rectangle indicates the 1 s time interval that was used to compare the velocity of the larvae in (D). (D) Violin plots depicting the velocity of AB, crystal, and Dc larvae during 1 s (3,002–3,003 s) following the first dark to light switch (see also Supplementary Figure 1D). Note that Dc increase their velocity significantly more and during a longer time period than AB wildtype and crystal zebrafish. One-way ANOVA followed by Tukey’s multiple comparisons test or Kruskal–Wallis test followed by Dunn’s multiple comparisons test was used to analyze differences in velocity between AB, crystal, and Dc; p > 0.05 is abbreviated as not significant (n.s.). (E) Violin plots depicting the time spent in the outer zone during the habituation (orange) and swimming (blue) phase is highest in Dc followed by AB, but lower in crystal larvae. (F) Violin plots show that thigmotaxis is increased in Dc relative to both AB and crystal zebrafish larvae during the light but not the dark phases. Two-way ANOVA followed by Šídák’s or Tukey’s multiple comparisons test was used to analyze differences in thigmotaxis between phases of the dark-light test in and between AB, crystal, and Dc; p > 0.05 is abbreviated as not significant (n.s.).